Hotspot

rrs

IRa · 197503-198623 · 1121 bp

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Pi 0.529
MarkerSeek score 73.90
Primer available yes
Label rrs

Feature Metadata

label_name
rrs
feature_type
rRNA
region
IRa
length_bp
1121
variable_sites
359
pi
0.529
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.60
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrs_p1 AGTTCACAATGGCGTTCACT CTCCGGATCGTTGCTTGTTA 45.0 50.0 58.0 58.0 2429 bp 0.5 59.7
rrs_p2 AGCGCCTGATATTGCAGAAA CTCCGGATCGTTGCTTGTTA 45.0 50.0 58.0 58.0 1836 bp 0.5 59.6
rrs_p3 CCAAAAAGTCCCGGGATGAA CTCCGGATCGTTGCTTGTTA 50.0 50.0 58.1 58.0 2060 bp 0.5 59.3
rrs_p4 AGTTCACAATGGCGTTCACT GAATCTCCGGATCGTTGCTT 45.0 50.0 58.0 58.1 2433 bp 0.5 59.3
rrs_p5 AGCGCCTGATATTGCAGAAA GAATCTCCGGATCGTTGCTT 45.0 50.0 58.0 58.1 1840 bp 0.5 59.2