Hotspot

orf333

SSC · 121551-122552 · 1002 bp

Back to Jenufa

Pi 0.458
MarkerSeek score 83.20
Primer available yes
Label orf333

Feature Metadata

label_name
orf333
feature_type
gene
region
SSC
length_bp
1002
variable_sites
430
pi
0.458
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf333_p1 CAGCACACCATCACTTAGCT AATTGCGGTGCATGGAAATG 50.0 45.0 57.9 58.0 2157 bp 0.5 59.1
orf333_p2 ACATCATGGCATGCTCAACT AATTGCGGTGCATGGAAATG 45.0 45.0 57.9 58.0 1999 bp 0.5 58.8
orf333_p3 GCAATGTGCTCACGGTTTTT AATTGCGGTGCATGGAAATG 45.0 45.0 58.1 58.0 1744 bp 0.5 58.7
orf333_p4 CAGCACACCATCACTTAGCT ACAAAAACCACCGATCCACA 50.0 45.0 57.9 57.9 1870 bp 0.5 58.7
orf333_p5 ACAACATCATGGCATGCTCA AATTGCGGTGCATGGAAATG 45.0 45.0 58.2 58.0 2002 bp 0.5 58.6