Hotspot

trnL(caa)-ycf1

SSC · 185089-186603 · 1515 bp

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Pi 0.485
MarkerSeek score 79.00
Primer available yes
Label trnL(caa)-ycf1

Feature Metadata

label_name
trnL(caa)-ycf1
feature_type
igs
region
SSC
length_bp
1515
variable_sites
572
pi
0.485
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.78
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL(caa)-ycf1_p1 AAAGCGTCGACTTTTGAGGT GGGCTATTGACGTAGGCAAA 45.0 50.0 58.1 58.0 1964 bp 0.5 59.3
trnL(caa)-ycf1_p2 AAACTGGTAGCGCGGTTAAT GGGCTATTGACGTAGGCAAA 45.0 50.0 57.9 58.0 2593 bp 0.5 58.9
trnL(caa)-ycf1_p3 AAAGCGTCGACTTTTGAGGT CCGCTTCTGTTCCTTGAGTT 45.0 50.0 58.1 58.1 2009 bp 0.5 58.7
trnL(caa)-ycf1_p4 CTGGTAGCGCGGTTAATGAT GGGCTATTGACGTAGGCAAA 50.0 50.0 58.1 58.0 2590 bp 0.5 58.7
trnL(caa)-ycf1_p5 AAACTGGTAGCGCGGTTAAT CCGCTTCTGTTCCTTGAGTT 45.0 50.0 57.9 58.1 2638 bp 0.5 58.2