Hotspot

trnE(uuc)-trnF(gaa)

SSC · 146085-146553 · 469 bp

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Pi 0.442
MarkerSeek score 85.20
Primer available yes
Label trnE(uuc)-trnF(gaa)

Feature Metadata

label_name
trnE(uuc)-trnF(gaa)
feature_type
igs
region
SSC
length_bp
469
variable_sites
188
pi
0.442
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE(uuc)-trnF(gaa)_p1 ATTCGAATCCCCGTTTCCTC CGGGATAGCTCAGTGGTAGA 50.0 55.0 57.7 58.0 599-719 bp 1.0 89.2
trnE(uuc)-trnF(gaa)_p2 ATTCGAATCCCCGTTTCCTC CTCAGTGGTAGAGCAGAGGA 50.0 55.0 57.7 57.9 591-711 bp 1.0 88.3
trnE(uuc)-trnF(gaa)_p3 ATTCGAATCCCCGTTTCCTC AGTGGTAGAGCAGAGGACTG 50.0 55.0 57.7 58.2 588-708 bp 1.0 88.2
trnE(uuc)-trnF(gaa)_p4 ATTCGAATCCCCGTTTCCTC CAGTGGTAGAGCAGAGGACT 50.0 55.0 57.7 58.2 589-709 bp 1.0 88.2
trnE(uuc)-trnF(gaa)_p5 ATTCGAATCCCCGTTTCCTC GTGGTAGAGCAGAGGACTGA 50.0 55.0 57.7 58.2 587-707 bp 1.0 88.1