Hotspot

rps4-trnS(gga)

SSC · 128378-128974 · 597 bp

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Pi 0.435
MarkerSeek score 83.10
Primer available yes
Label rps4-trnS(gga)

Feature Metadata

label_name
rps4-trnS(gga)
feature_type
igs
region
SSC
length_bp
597
variable_sites
243
pi
0.435
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnS(gga)_p1 CACCAGGTCAACACGGTTTA CCGTACCTTAAACCACTCGG 50.0 55.0 58.0 58.0 1244 bp 0.5 59.6
rps4-trnS(gga)_p2 CACCAGGTCAACACGGTTTA TATCCACTTATCGCCCTCGT 50.0 50.0 58.0 58.0 1780 bp 0.5 59.6
rps4-trnS(gga)_p3 CACCAGGTCAACACGGTTTA GCAGCTTCTTTGGCTTTAGC 50.0 50.0 58.0 58.0 1952 bp 0.5 59.6
rps4-trnS(gga)_p4 CACCAGGTCAACACGGTTTA TCCATCAACCAGAAGGAGGT 50.0 50.0 58.0 58.0 1882 bp 0.5 59.3
rps4-trnS(gga)_p5 CACCAGGTCAACACGGTTTA GCTTTAGCTGGCGGAACTAT 50.0 50.0 58.0 58.0 1940 bp 0.5 59.3