Hotspot

trnY(gua)-psbK

LSC · 47162-47957 · 796 bp

Back to Jenufa

Pi 0.488
MarkerSeek score 83.60
Primer available yes
Label trnY(gua)-psbK

Feature Metadata

label_name
trnY(gua)-psbK
feature_type
igs
region
LSC
length_bp
796
variable_sites
371
pi
0.488
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY(gua)-psbK_p1 CTCGTGAAGGTGGCGAAATA CACCCCCACCGTTTAGTTTT 50.0 50.0 58.0 58.3 2091 bp 0.5 57.5
trnY(gua)-psbK_p2 TACGTTGGTTCGAATCCGAC CACCCCCACCGTTTAGTTTT 50.0 50.0 58.0 58.3 1397 bp 0.5 57.4
trnY(gua)-psbK_p3 AGTCCTGAATTAGCTGCTGC CACCCCCACCGTTTAGTTTT 50.0 50.0 58.0 58.3 2050 bp 0.5 57.3
trnY(gua)-psbK_p4 TTTGCCTACGTTGGTTCGAA CACCCCCACCGTTTAGTTTT 45.0 50.0 58.0 58.3 1403 bp 0.5 57.2
trnY(gua)-psbK_p5 TTCAGAACACCCCCTGGTAT CACCCCCACCGTTTAGTTTT 50.0 50.0 57.9 58.3 1721 bp 0.5 57.1