Hotspot

rpl16-rps3

LSC · 89892-90099 · 208 bp

Back to Jatropha

Pi 0.0632

MarkerSeek score 73.80

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 12
pi: 0.0632
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TAAAAACCCAACGAGTCGCA	GGGTAGAGTTCCTTTACAAACCA	45.0	43.5	58.0	58.0	338-356 bp	1.0	62.2
rpl16-rps3_p2	TAAAAACCCAACGAGTCGCA	AGGGTAGAGTTCCTTTACAAACC	45.0	43.5	58.0	57.8	339-357 bp	1.0	60.5
rpl16-rps3_p3	TAAAAACCCAACGAGTCGCA	GGGTAGAGTTCCTTTACAAACC	45.0	45.5	58.0	56.4	338-356 bp	1.0	57.2
rpl16-rps3_p4	TAAAAACCCAACGAGTCGCA	TCCTTTACAAACCATTCAAGCT	45.0	36.4	58.0	56.2	329-347 bp	1.0	55.8
rpl16-rps3_p5	CCAACGAGTCGCACACTAAG	GGGTAGAGTTCCTTTACAAACCA	55.0	43.5	58.9	58.0	331-349 bp	1.0	55.1