Hotspot

petD-rpoA

LSC · 84305-84515 · 211 bp

Back to Jatropha

Pi 0.0429

MarkerSeek score 71.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 211
variable_sites: 9
pi: 0.0429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GATTGGTACTGCAGTAGCCC	TCGCATAGACGATGTAAAACA	55.0	38.1	58.0	55.5	399 bp	1.0	53.4
petD-rpoA_p2	GCAGTAGCCCTTTGGTTAGG	TCGCATAGACGATGTAAAACA	55.0	38.1	58.2	55.5	389 bp	1.0	51.8
petD-rpoA_p3	GGTACTGCAGTAGCCCTTTG	TCGCATAGACGATGTAAAACA	55.0	38.1	58.3	55.5	395 bp	1.0	51.7
petD-rpoA_p4	GTACTGCAGTAGCCCTTTGG	TCGCATAGACGATGTAAAACA	55.0	38.1	58.3	55.5	394 bp	1.0	51.7
petD-rpoA_p5	GATTGGTACTGCAGTAGCCC	TTCGCATAGACGATGTAAAACA	55.0	36.4	58.0	56.2	400 bp	1.0	51.4