Hotspot

ndhC-trnV-UAC

LSC · 55406-56380 · 975 bp

Back to Jatropha

Pi 0.0528

MarkerSeek score 75.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 38
pi: 0.0528
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCTTTGCTAATCGGGGATA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	56.8	57.8	939-1119 bp	1.0	75.4
ndhC-trnV-UAC_p2	CTCCGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	57.9	50.0	58.3	57.8	945-1125 bp	1.0	75.0
ndhC-trnV-UAC_p3	TTCTCCGGCCCTTTGCTAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	59.4	57.8	947-1127 bp	1.0	74.3
ndhC-trnV-UAC_p4	CCCTTTGCTAATCGGGGATAA	AGAAGGTCTACGGTTCGAGT	47.6	50.0	57.5	57.8	939-1119 bp	1.0	73.4
ndhC-trnV-UAC_p5	CCCTTTGCTAATCGGGGATA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	56.8	57.5	938-1118 bp	1.0	72.7