Hotspot

psbZ-trnG-GCC

LSC · 40522-41370 · 849 bp

Back to Jatropha

Pi 0.0419

MarkerSeek score 77.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 33
pi: 0.0419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	1000-1310 bp	1.0	78.0
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	1003-1313 bp	1.0	75.7
psbZ-trnG-GCC_p3	TCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	1001-1311 bp	1.0	75.7
psbZ-trnG-GCC_p4	TCTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.4	57.8	1004-1314 bp	1.0	73.9
psbZ-trnG-GCC_p5	TTCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.6	57.8	1002-1312 bp	1.0	73.5