Hotspot

trnE-UUC-trnT_GGU

LSC · 34339-35479 · 1141 bp

Back to Jatropha

Pi 0.0214

MarkerSeek score 74.10

Primer available yes

Label trnE-UUC-trnT_GGU

Feature Metadata

label_name: trnE-UUC-trnT_GGU
feature_type: igs
region: LSC
length_bp: 1141
variable_sites: 22
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT_GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	1217-1244 bp	1.0	83.3
trnE-UUC-trnT_GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	1196-1223 bp	1.0	83.2
trnE-UUC-trnT_GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	1196-1223 bp	1.0	82.8
trnE-UUC-trnT_GGU_p4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	1175-1202 bp	1.0	82.7
trnE-UUC-trnT_GGU_p5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	1193-1220 bp	1.0	82.7