Hotspot

trnR-UCU-atpA

LSC · 10559-11146 · 588 bp

Back to Jatropha

Pi 0.0521

MarkerSeek score 71.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 588
variable_sites: 17
pi: 0.0521
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	TCACCGAAGAAGCAGAAAGC	47.6	50.0	57.3	58.5	450-690 bp	1.0	68.9
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AGGAACTGAAGGAGCGTTTT	47.6	45.0	57.3	57.4	411-651 bp	1.0	67.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	ACATTCACCGAAGAAGCAGA	47.6	45.0	57.3	57.2	454-694 bp	1.0	66.1
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	TCACCGAAGAAGCAGAAAGC	47.6	50.0	56.9	58.5	485-725 bp	1.0	65.9
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	GGAACTGAAGGAGCGTTTTC	47.6	50.0	57.3	57.0	410-650 bp	1.0	65.3