Hotspot

trnS-GCU-trnG-GCC

LSC · 7552-8890 · 1339 bp

Back to Jatropha

Pi 0.0736
MarkerSeek score 87.40
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1339
variable_sites
74
pi
0.0736
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.75
markerseek_score
87.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA GAATATGAACCGAGGGATCCC 50.0 52.4 58.0 57.7 1172-1465 bp 1.0 77.8
trnS-GCU-trnG-GCC_p2 CAATCCGACGCTTTAGTCCA GGAATATGAACCGAGGGATCC 50.0 52.4 58.0 57.7 1173-1466 bp 1.0 77.8
trnS-GCU-trnG-GCC_p3 AATCCGACGCTTTAGTCCAC GAATATGAACCGAGGGATCCC 50.0 52.4 58.0 57.7 1171-1464 bp 1.0 77.8
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC GGAATATGAACCGAGGGATCC 50.0 52.4 58.0 57.7 1172-1465 bp 1.0 77.8
trnS-GCU-trnG-GCC_p5 AAATTCCAAGTCATCGCCGA GAATATGAACCGAGGGATCCC 45.0 52.4 57.9 57.7 1248-1541 bp 1.0 76.7