Hotspot

ycf1

IRa · 133013-138922 · 5910 bp

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Pi 0.0297

MarkerSeek score 69.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 5910
variable_sites: 624
pi: 0.0297
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCGGAACAAGCAAATCCAA	CTGTTGATGGATATGGCGGG	45.0	55.0	58.0	58.5	80-1388 bp	0.5	68.1
ycf1_p2	TAACGTCTTAAGGCCGCATC	GCGGGGGAGATCTCTATCAA	50.0	55.0	58.1	58.4	80-812 bp	0.428571	62.2
ycf1_p3	TTCAATCTTACGAGCCGACG	GCGGGGGAGATCTCTATCAA	50.0	55.0	58.1	58.4	80-1035 bp	0.428571	61.9
ycf1_p4	TCCGGAACAAGCAAATCCAA	GCGGGGGAGATCTCTATCAA	45.0	55.0	58.0	58.4	80-1373 bp	0.428571	61.8
ycf1_p5	TTCCGGAACAAGCAAATCCA	GCGGGGGAGATCTCTATCAA	45.0	55.0	58.0	58.4	80-1374 bp	0.428571	61.8