Hotspot

ndhF-rpl32

SSC · 121817-122310 · 494 bp

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Pi 0.036

MarkerSeek score 76.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 494
variable_sites: 64
pi: 0.036
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCACATACGACGAAGATT	AGGTCGATGGGAAAATGAGC	50.0	50.0	58.1	58.0	80-1063 bp	0.428571	63.5
ndhF-rpl32_p2	GCCCACATACGACGAAGATT	GTCGATGGGAAAATGAGCCT	50.0	50.0	58.1	58.0	80-1061 bp	0.428571	63.5
ndhF-rpl32_p3	CAAGAGGTCGTGTGAACCAA	AGGTCGATGGGAAAATGAGC	50.0	50.0	58.1	58.0	80-1452 bp	0.428571	62.1
ndhF-rpl32_p4	CAAGAGGTCGTGTGAACCAA	GTCGATGGGAAAATGAGCCT	50.0	50.0	58.1	58.0	80-1450 bp	0.428571	62.1
ndhF-rpl32_p5	ACAAACGCCTTTTGACAAGC	GTCGATGGGAAAATGAGCCT	45.0	50.0	58.1	58.0	80-1478 bp	0.428571	61.8