Hotspot

trnV-UAC-ndhC

LSC · 55517-57039 · 1523 bp

Back to Jasminum

Pi 0.0289

MarkerSeek score 65.30

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 1523
variable_sites: 94
pi: 0.0289
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	GCGTGTTGGGTCTTTGAAAC	GGCTTGAGATTATTAGAAATGCCC	50.0	41.7	58.2	58.0	80-1675 bp	1.0	57.2
trnV-UAC-ndhC_p2	GCGTGTTGGGTCTTTGAAAC	AATGGGAATAAGGCTTGAGA	50.0	40.0	58.2	54.0	80-1686 bp	1.0	57.0
trnV-UAC-ndhC_p3	GCGTGTTGGGTCTTTGAAAC	TGGGAATAAGGCTTGAGATT	50.0	40.0	58.2	54.0	80-1684 bp	1.0	57.0
trnV-UAC-ndhC_p4	AGAAGGTCTACGGTTCGAGT	GGCTTGAGATTATTAGAAATGCCC	50.0	41.7	57.8	58.0	80-1611 bp	0.928571	53.8
trnV-UAC-ndhC_p5	AGAAGGTCTACGGTTCGAGT	TGGGAATAAGGCTTGAGATT	50.0	40.0	57.8	54.0	80-1620 bp	0.928571	53.6