Hotspot

rps2-rpoC2

LSC · 17859-19076 · 1218 bp

Back to Jasminum

Pi 0.198

MarkerSeek score 81.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 625
pi: 0.198
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CTTCGTGCTGATCAACGAGA	GATTGGTTGAAGGGCCTGAA	50.0	50.0	58.0	58.1	1767-2190 bp	0.928571	88.4
rps2-rpoC2_p2	TCTTCGTGCTGATCAACGAG	GATTGGTTGAAGGGCCTGAA	50.0	50.0	58.0	58.1	1768-2191 bp	0.928571	88.4
rps2-rpoC2_p3	CTTCGTGCTGATCAACGAGA	GGGTCGAATCGATTGGTTGA	50.0	50.0	58.0	58.0	1777-2200 bp	0.785714	83.1
rps2-rpoC2_p4	TCTTCGTGCTGATCAACGAG	GGGTCGAATCGATTGGTTGA	50.0	50.0	58.0	58.0	1778-2201 bp	0.785714	83.1
rps2-rpoC2_p5	CTTATCGCAGCCCGTACTAC	GGGTCGAATCGATTGGTTGA	55.0	50.0	58.0	58.0	1529-1982 bp	0.785714	82.9