Hotspot

atpF

LSC · 34263-35694 · 1432 bp

Back to Jansenella

Pi 0.00281

MarkerSeek score 60.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1432
variable_sites: 4
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACGAGCGAAGTAAGTGATCG	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.8	57.9	1595-1601 bp	1.0	78.2
atpF_p2	ACGAGCGAAGTAAGTGATCG	CGAAGGGTTGCCATTAGTGT	50.0	50.0	57.8	58.2	1594-1600 bp	1.0	77.7
atpF_p3	CGAGCGAAGTAAGTGATCGA	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.5	57.9	1594-1600 bp	1.0	76.0
atpF_p4	ACGAGCGAAGTAAGTGATCG	GACTCGAAGGGTTGCCATTA	50.0	50.0	57.8	57.6	1598-1604 bp	1.0	75.9
atpF_p5	ACGAGCGAAGTAAGTGATCG	CTCGAAGGGTTGCCATTAGT	50.0	50.0	57.8	57.6	1596-1602 bp	1.0	75.9