Hotspot

rpl32-trnL-UAG

SSC · 107853-108356 · 504 bp

Back to Jansenella

Pi 0.00794
MarkerSeek score 61.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
504
variable_sites
4
pi
0.00794
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAACGAGCATCCAAAACCAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.2 57.9 595 bp 1.0 73.2
rpl32-trnL-UAG_p2 CAACGAGCATCCAAAACCAA AGCAGCGTGTCTACCAATTT 45.0 45.0 57.2 57.8 588 bp 1.0 72.7
rpl32-trnL-UAG_p3 CAACGAGCATCCAAAACCAA GCTTGAGCACTGCTTCCTAA 45.0 50.0 57.2 58.2 609 bp 1.0 72.7
rpl32-trnL-UAG_p4 CAACGAGCATCCAAAACCAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.2 57.8 594 bp 1.0 72.6
rpl32-trnL-UAG_p5 CAACGAGCATCCAAAACCAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.2 57.8 593 bp 1.0 72.6