Hotspot

trnG-UCC-trnM-CAU

LSC · 13491-15477 · 1987 bp

Back to Jansenella

Pi 0.00151

MarkerSeek score 58.60

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1987
variable_sites: 3
pi: 0.00151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	ACTCCTGTCTTACCAAGGCA	38.1	50.0	55.5	58.3	2129 bp	1.0	49.7
trnG-UCC-trnM-CAU_p2	ACGAATCACACTTTTACCACT	GACTCCTGTCTTACCAAGGC	38.1	55.0	55.5	57.6	2130 bp	1.0	48.7
trnG-UCC-trnM-CAU_p3	AGAACGAATCACACTTTTACCAC	ACTCCTGTCTTACCAAGGCA	39.1	50.0	57.3	58.3	2132 bp	1.0	48.6
trnG-UCC-trnM-CAU_p4	GAACGAATCACACTTTTACCACT	ACTCCTGTCTTACCAAGGCA	39.1	50.0	57.3	58.3	2131 bp	1.0	48.6
trnG-UCC-trnM-CAU_p5	AACGAATCACACTTTTACCACT	ACTCCTGTCTTACCAAGGCA	36.4	50.0	56.2	58.3	2130 bp	1.0	47.7