Hotspot

psbB

LSC · 74863-76389 · 1527 bp

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Pi 0.00263

MarkerSeek score 55.50

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 4
pi: 0.00263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	CGACTGAACAAGAGTGGTCC	AGGGCGAAAGAAAGATACCA	55.0	45.0	58.2	56.5	1654-1661 bp	1.0	67.1
psbB_p2	CGACTGAACAAGAGTGGTCC	AAGGGCGAAAGAAAGATACCA	55.0	42.9	58.2	57.3	1655-1662 bp	1.0	65.1
psbB_p3	CGACTGAACAAGAGTGGTCC	AGGGCGAAAGAAAGATACCAA	55.0	42.9	58.2	57.3	1654-1661 bp	1.0	65.1
psbB_p4	CGACTGAACAAGAGTGGTCC	AAGGGCGAAAGAAAGATACCAA	55.0	40.9	58.2	58.0	1655-1662 bp	1.0	62.6
psbB_p5	CGACTGAACAAGAGTGGTCC	AGGGCGAAAGAAAGATACCAAA	55.0	40.9	58.2	58.0	1654-1661 bp	1.0	62.6