Hotspot

ndhB-trnL-CAA

IRa · 145146-145684 · 539 bp

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Pi 0

MarkerSeek score 34.50

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 539
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	GCAATTGCTCAGGATAGCCT	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.0	58.0	598 bp	1.0	79.8
ndhB-trnL-CAA_p2	GCAATTGCTCAGGATAGCCT	CTCGCGTGTCTACCATTTCA	50.0	50.0	58.0	58.0	596 bp	1.0	79.8
ndhB-trnL-CAA_p3	GCAATTGCTCAGGATAGCCT	TACCATTTCACCACCAAGGC	50.0	50.0	58.0	58.1	586 bp	1.0	79.2
ndhB-trnL-CAA_p4	GCAATTGCTCAGGATAGCCT	GCGTGTCTACCATTTCACCA	50.0	50.0	58.0	58.2	593 bp	1.0	78.3
ndhB-trnL-CAA_p5	GCAATTGCTCAGGATAGCCT	TCTCGCGTGTCTACCATTTC	50.0	50.0	58.0	57.7	597 bp	1.0	77.6