Hotspot

trnR-ACG-trnN-GUU

SSC · 109830-110407 · 578 bp

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Pi 0.00692

MarkerSeek score 62.30

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: SSC
length_bp: 578
variable_sites: 4
pi: 0.00692
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	670 bp	1.0	77.5
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGGAACGGGAATTGAACTC	55.0	50.0	57.8	57.8	755 bp	1.0	77.1
trnR-ACG-trnN-GUU_p3	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	707 bp	1.0	76.9
trnR-ACG-trnN-GUU_p4	GAACAGATCCAGTGGAGACG	TGGGAACGGGAATTGAACTC	55.0	50.0	57.7	57.8	792 bp	1.0	76.6
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	57.8	57.7	674 bp	1.0	76.6