Hotspot

ycf15-ndhB

IRb · 95931-96898 · 968 bp

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Pi 0.00413

MarkerSeek score 61.10

Primer available yes

Label ycf15-ndhB

Feature Metadata

label_name: ycf15-ndhB
feature_type: igs
region: IRb
length_bp: 968
variable_sites: 4
pi: 0.00413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-ndhB_p1	AGGAGAAGGAAGGCTGGAAT	GCAATTGCTCAGGATAGCCT	50.0	50.0	58.0	58.0	1052 bp	1.0	80.0
ycf15-ndhB_p2	GGAGAAGGAAGGCTGGAATG	GCAATTGCTCAGGATAGCCT	55.0	50.0	58.0	58.0	1051 bp	1.0	79.7
ycf15-ndhB_p3	GAAAGGAGAAGGAAGGCTGG	GCAATTGCTCAGGATAGCCT	55.0	50.0	57.9	58.0	1055 bp	1.0	79.2
ycf15-ndhB_p4	TCTTTCGGATAGGCCCTGAA	GCAATTGCTCAGGATAGCCT	50.0	50.0	58.1	58.0	1072 bp	1.0	79.1
ycf15-ndhB_p5	TTCTCTTTCGGATAGGCCCT	GCAATTGCTCAGGATAGCCT	50.0	50.0	57.8	58.0	1075 bp	1.0	78.7