Hotspot

rbcL-accD

LSC · 58515-59270 · 756 bp

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Pi 0.00265

MarkerSeek score 60.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 2
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	CTGCGTTCGAACTCCTTCTT	50.0	50.0	57.9	58.2	902-925 bp	1.0	77.5
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	TTATTTAGCCCACACCTGCG	50.0	50.0	57.9	58.3	917-940 bp	1.0	77.2
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	TTCTTGCCCCCTAATTTGCA	50.0	45.0	57.9	57.7	843-866 bp	1.0	76.7
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	GGATCAAGACTGCCCATTGA	50.0	50.0	57.9	57.6	938-961 bp	1.0	75.9
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	AGGATCAAGACTGCCCATTG	50.0	50.0	57.9	57.6	939-962 bp	1.0	75.8