Hotspot

atpB-rbcL

LSC · 56271-57080 · 810 bp

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Pi 0.00247

MarkerSeek score 60.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 2
pi: 0.00247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GACCGATGATTTGGACGACA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	944-951 bp	1.0	72.0
atpB-rbcL_p2	GACCGATGATTTGGACGACA	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	943-950 bp	1.0	71.7
atpB-rbcL_p3	GACCGATGATTTGGACGACA	CCTTGGTTTGGTACTCAGGAG	50.0	52.4	58.0	57.9	992-999 bp	1.0	71.6
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	898-905 bp	1.0	71.3
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	897-904 bp	1.0	71.1