Hotspot

atpH-atpI

LSC · 13736-14906 · 1171 bp

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Pi 0.00512

MarkerSeek score 61.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1171
variable_sites: 6
pi: 0.00512
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCCTCTACAGCTTGACCC	TACCTGTCATGCTCCTTGGA	55.0	50.0	58.1	58.0	1390-1397 bp	1.0	78.8
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TACCTGTCATGCTCCTTGGA	50.0	50.0	57.8	58.0	1322-1329 bp	1.0	78.3
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TACCTGTCATGCTCCTTGGA	50.0	50.0	58.3	58.0	1317-1324 bp	1.0	77.9
atpH-atpI_p4	AATCCAGCAGCAATAACGGA	TACCTGTCATGCTCCTTGGA	45.0	50.0	57.6	58.0	1319-1326 bp	1.0	76.6
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TACCTGTCATGCTCCTTGGA	45.0	50.0	57.6	58.0	1318-1325 bp	1.0	76.6