Hotspot

trnK-rps16

LSC · 4322-5020 · 699 bp

Back to Jaltomata

Pi 0.00429

MarkerSeek score 61.20

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 3
pi: 0.00429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AAAGCCGAGTACTCTACCGT	TTGAAAAAGGGGCTCAACCT	50.0	45.0	58.2	57.5	831-834 bp	1.0	75.2
trnK-rps16_p2	AAGCCGAGTACTCTACCGTT	TTGAAAAAGGGGCTCAACCT	50.0	45.0	58.2	57.5	830-833 bp	1.0	75.2
trnK-rps16_p3	TCAGTCGTGGTCTTCCAAAC	TTGAAAAAGGGGCTCAACCT	50.0	45.0	57.8	57.5	916-919 bp	1.0	74.6
trnK-rps16_p4	CAGTCGTGGTCTTCCAAACT	TTGAAAAAGGGGCTCAACCT	50.0	45.0	57.8	57.5	915-918 bp	1.0	74.6
trnK-rps16_p5	AAAAGACACTAGCCGCACTT	TTGAAAAAGGGGCTCAACCT	45.0	45.0	57.7	57.5	852-855 bp	1.0	74.5