Hotspot

ycf1

SSC · 121800-126872 · 5073 bp

Back to Jacobaea

Pi 0.386

MarkerSeek score 75.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5073
variable_sites: 1743
pi: 0.386
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTCTTCCGCCTGTGAAAA	GACCGTCTCAAGGAAATCCC	45.0	55.0	57.4	58.0	904 bp	0.5	54.7
ycf1_p2	TCTTCTTCCGCCTGTGAAAA	GGACCGTCTCAAGGAAATCC	45.0	55.0	57.4	58.0	905 bp	0.5	54.7
ycf1_p3	TCTTCTTCCGCCTGTGAAAA	GGGACCGTCTCAAGGAAATC	45.0	55.0	57.4	58.0	906 bp	0.5	54.7
ycf1_p4	TTCTTCTTCCGCCTGTGAAA	GACCGTCTCAAGGAAATCCC	45.0	55.0	57.4	58.0	905 bp	0.5	54.7
ycf1_p5	TTCTTCTTCCGCCTGTGAAA	GGACCGTCTCAAGGAAATCC	45.0	55.0	57.4	58.0	906 bp	0.5	54.7