Hotspot

ndhI

SSC · 117235-117735 · 501 bp

Back to Jacobaea

Pi 0.474

MarkerSeek score 80.20

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 229
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGACCACGGAAGTTACTCCA	AGCCGAGTGTGAACGATTAC	50.0	50.0	58.0	58.0	1684 bp	0.5	59.9
ndhI_p2	AGACCACGGAAGTTACTCCA	GGTTCTGCAATAGGGATGGG	50.0	55.0	58.0	58.0	1846 bp	0.5	59.9
ndhI_p3	AGACCACGGAAGTTACTCCA	TTTGTGACCTTTGAGGAGCC	50.0	50.0	58.0	58.0	1890 bp	0.5	59.8
ndhI_p4	AGACCACGGAAGTTACTCCA	GACCTTTGAGGAGCCGTATG	50.0	55.0	58.0	58.1	1885 bp	0.5	59.5
ndhI_p5	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1379 bp	0.5	59.5