Hotspot

ndhH

SSC · 120044-121222 · 1179 bp

Back to Jacobaea

Pi 0.491

MarkerSeek score 79.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1179
variable_sites: 469
pi: 0.491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CCAATGGTCCTGCGTATTCA	CGCTTGCTGGCTTATTTGTC	50.0	50.0	58.0	58.1	1572 bp	0.5	58.9
ndhH_p2	CCAATGGTCCTGCGTATTCA	GGGAGCCGTTTGAGTATGTT	50.0	50.0	58.0	57.9	2010 bp	0.5	58.8
ndhH_p3	GTTGTTGTATACCCGCGGAT	CGCTTGCTGGCTTATTTGTC	50.0	50.0	58.1	58.1	1542 bp	0.5	58.8
ndhH_p4	CCTGACATAAGAAGGCCGAC	CGCTTGCTGGCTTATTTGTC	55.0	50.0	58.1	58.1	1807 bp	0.5	58.7
ndhH_p5	GACATAAGAAGGCCGACAGG	CGCTTGCTGGCTTATTTGTC	55.0	50.0	58.1	58.1	1804 bp	0.5	58.7