Hotspot

ndhD

SSC · 113695-115215 · 1521 bp

Back to Jacobaea

Pi 0.457

MarkerSeek score 82.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 644
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGAGAACCACGTGAATCACT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	1936 bp	0.5	59.3
ndhD_p2	CGAGAACCACGTGAATCACT	ACCTTATTTTGGGGCACAGG	50.0	50.0	57.9	58.1	1748 bp	0.5	59.0
ndhD_p3	CGAGAACCACGTGAATCACT	CCTTATTTTGGGGCACAGGT	50.0	50.0	57.9	58.1	1747 bp	0.5	59.0
ndhD_p4	TCGTGTGATTAGCCTAGGGT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.8	58.0	2309 bp	0.5	58.6
ndhD_p5	CGAGAACCACGTGAATCACT	CAGAAACCCCTACTTGAGCC	50.0	55.0	57.9	57.9	1803 bp	0.5	58.5