Hotspot

ccsA

SSC · 112458-113426 · 969 bp

Back to Jacobaea

Pi 0.446

MarkerSeek score 83.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 400
pi: 0.446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1564 bp	0.5	59.6
ccsA_p2	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1563 bp	0.5	59.6
ccsA_p3	GTGAAATCGGTAGACACGCT	AGTGATTCACGTGGTTCTCG	50.0	50.0	58.0	57.9	1247 bp	0.5	59.4
ccsA_p4	TGAAATCGGTAGACACGCTG	AGTGATTCACGTGGTTCTCG	50.0	50.0	58.0	57.9	1246 bp	0.5	59.4
ccsA_p5	TCTTTCTCGGTAAGGGCAGA	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.1	58.0	2114 bp	0.5	59.2