Hotspot

ndhD-psaC

SSC · 115216-115311 · 96 bp

Back to Jacobaea

Pi 0.477

MarkerSeek score 77.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 96
variable_sites: 42
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CTCCCGCCGTGTACAAAATA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	760 bp	0.5	59.8
ndhD-psaC_p2	TTCCAAAGCCACAGGATACG	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	870 bp	0.5	59.0
ndhD-psaC_p3	TCCTCCCCACATGCATAGAA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.1	58.0	711 bp	0.5	59.0
ndhD-psaC_p4	TTCCTCCCCACATGCATAGA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.1	58.0	712 bp	0.5	59.0
ndhD-psaC_p5	ACCATATAACCCGACACCCA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.1	58.0	807 bp	0.5	58.9