Hotspot

ccsA-ndhD

SSC · 113427-113694 · 268 bp

Back to Jacobaea

Pi 0.428

MarkerSeek score 76.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 268
variable_sites: 107
pi: 0.428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACCCAAGGCTTTGCTACTTC	CTCCCGGCCAACGTAATTTA	50.0	50.0	58.1	58.0	1533 bp	0.5	59.1
ccsA-ndhD_p2	CCCAAGGCTTTGCTACTTCA	CTCCCGGCCAACGTAATTTA	50.0	50.0	58.1	58.0	1532 bp	0.5	59.1
ccsA-ndhD_p3	ACCCAAGGCTTTGCTACTTC	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.1	58.0	1057 bp	0.5	59.0
ccsA-ndhD_p4	CCCAAGGCTTTGCTACTTCA	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.1	58.0	1056 bp	0.5	59.0
ccsA-ndhD_p5	ACCCAAGGCTTTGCTACTTC	CATGGCTTTATTGGTGCTGC	50.0	50.0	58.1	58.1	1405 bp	0.5	58.7