Hotspot

rpl16

LSC · 81883-83304 · 1422 bp

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Pi 0.0191
MarkerSeek score 58.90
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1422
variable_sites
40
pi
0.0191
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ACACATCAATTCTCGAGCCC AAGTCCAAATTGCAGGTCGT 50.0 45.0 58.0 58.0 1902-1921 bp 1.0 82.5
rpl16_p2 ACACATCAATTCTCGAGCCC GCTACAAACCATTCGGGCTA 50.0 50.0 58.0 58.0 1829-1848 bp 1.0 82.5
rpl16_p3 TTCTGACCTTTCTAGGGGCA AAGTCCAAATTGCAGGTCGT 50.0 45.0 58.0 58.0 2007-2026 bp 1.0 82.4
rpl16_p4 TTCTGACCTTTCTAGGGGCA GCTACAAACCATTCGGGCTA 50.0 50.0 58.0 58.0 1934-1953 bp 1.0 82.3
rpl16_p5 CAGCTCTGACCACTTCTGAC AAGTCCAAATTGCAGGTCGT 55.0 45.0 57.9 58.0 2020-2039 bp 1.0 82.0