Hotspot

ndhF

SSC · 110741-112969 · 2229 bp

Pi 0.0123

MarkerSeek score 50.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 41
pi: 0.0123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	CAAAGGGGTAAGATCCGGTG	50.0	55.0	58.0	58.0	2587-2615 bp	1.0	81.7
ndhF_p2	ACAGGAACAAGAGGGATCCA	AAAGGGGTAAGATCCGGTGA	50.0	50.0	58.0	58.0	2586-2614 bp	1.0	81.7
ndhF_p3	ACAGGAACAAGAGGGATCCA	AAGGGGTAAGATCCGGTGAA	50.0	50.0	58.0	58.0	2585-2613 bp	1.0	81.7
ndhF_p4	ACAGGAACAAGAGGGATCCA	TCAAAGGGGTAAGATCCGGT	50.0	50.0	58.0	58.0	2588-2616 bp	1.0	81.7
ndhF_p5	AAGAGGGATCCACCGAAGAA	CAAAGGGGTAAGATCCGGTG	50.0	55.0	58.0	58.0	2579-2607 bp	1.0	81.7