Hotspot

trnV-GAC-rps12

IRa · 138947-140602 · 1656 bp

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Pi 0.00265
MarkerSeek score 62.90
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1656
variable_sites
6
pi
0.00265
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 ACTCACACTGGGTTTAGGGA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 1742-1788 bp 1.0 79.4
trnV-GAC-rps12_p2 GAGGGGAGCAAAGCCAAATA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 1767-1813 bp 1.0 79.1
trnV-GAC-rps12_p3 GGGGAGCAAAGCCAAATAGA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 1765-1811 bp 1.0 79.1
trnV-GAC-rps12_p4 AGGGGAGCAAAGCCAAATAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 1766-1812 bp 1.0 79.1
trnV-GAC-rps12_p5 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1706-1752 bp 1.0 79.0