Hotspot

rps12-trnV-GAC

IRb · 99066-100721 · 1656 bp

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Pi 0.00245
MarkerSeek score 62.80
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1656
variable_sites
6
pi
0.00245
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA ACTCACACTGGGTTTAGGGA 45.0 50.0 57.7 57.9 1742-1788 bp 1.0 79.4
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA GAGGGGAGCAAAGCCAAATA 45.0 50.0 57.7 57.9 1767-1813 bp 1.0 79.1
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA GGGGAGCAAAGCCAAATAGA 45.0 50.0 57.7 57.9 1765-1811 bp 1.0 79.1
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA AGGGGAGCAAAGCCAAATAG 45.0 50.0 57.7 57.9 1766-1812 bp 1.0 79.1
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1706-1752 bp 1.0 79.0