Hotspot

trnP-UGG-psaJ

LSC · 67254-67684 · 431 bp

Back to Ixora

Pi 0.0174
MarkerSeek score 68.40
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
431
variable_sites
11
pi
0.0174
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC CCAAAGCTCCGAACCATAGT 50.0 50.0 58.1 57.6 543-553 bp 1.0 79.0
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA CCAAAGCTCCGAACCATAGT 50.0 50.0 58.1 57.6 542-552 bp 1.0 79.0
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC CCAAAGCTCCGAACCATAGT 50.0 50.0 58.1 57.6 554-564 bp 1.0 78.9
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA CCAAAGCTCCGAACCATAGT 50.0 50.0 58.1 57.6 553-563 bp 1.0 78.9
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG CCAAAGCTCCGAACCATAGT 50.0 50.0 58.1 57.6 556-566 bp 1.0 78.9