Hotspot

petA-psbJ

LSC · 63702-64609 · 908 bp

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Pi 0.0147
MarkerSeek score 69.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
908
variable_sites
20
pi
0.0147
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC CATATTCCGGATTGGGCTCA 50.0 50.0 58.0 57.4 1047-1056 bp 1.0 77.4
petA-psbJ_p2 GAGAAGGTTCAATTGGCCGA CATATTCCGGATTGGGCTCA 50.0 50.0 58.2 57.4 961-970 bp 1.0 76.4
petA-psbJ_p3 TCGAGAAGGTTCAATTGGCC CATATTCCGGATTGGGCTCA 50.0 50.0 58.2 57.4 963-972 bp 1.0 76.4
petA-psbJ_p4 CATTACGTGTCCAAGGCCTT CATATTCCGGATTGGGCTCA 50.0 50.0 58.2 57.4 1044-1053 bp 1.0 76.2
petA-psbJ_p5 AGAAGGTTCAATTGGCCGAA CATATTCCGGATTGGGCTCA 45.0 50.0 57.7 57.4 960-969 bp 1.0 75.6