Hotspot

accD-psaI

LSC · 59216-59911 · 696 bp

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Pi 0.0316
MarkerSeek score 76.70
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
696
variable_sites
30
pi
0.0316
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GACCTAATCGTACCGCGTAA CCATTGCAATTGCCGGAAAT 50.0 45.0 57.2 58.0 865-901 bp 1.0 79.1
accD-psaI_p2 GACCTAATCGTACCGCGTAA AAGAAGCCATTGCAATTGCC 50.0 45.0 57.2 57.9 871-907 bp 1.0 78.5
accD-psaI_p3 ATTTCAGTTCCACGCTTTCG CCATTGCAATTGCCGGAAAT 45.0 45.0 57.0 58.0 818-854 bp 1.0 77.7
accD-psaI_p4 ATTTCAGTTCCACGCTTTCG AAGAAGCCATTGCAATTGCC 45.0 45.0 57.0 57.9 824-860 bp 1.0 77.1
accD-psaI_p5 GACCTAATCGTACCGCGTAA GCCGGAAATACTAGGCCTAC 50.0 55.0 57.2 57.5 854-890 bp 1.0 75.8