Hotspot

ndhC-trnV-UAC

LSC · 50584-51816 · 1233 bp

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Pi 0.021
MarkerSeek score 64.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1233
variable_sites
36
pi
0.021
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAAGACTTGA GTTCGAGTCCGTATAGCCCT 33.3 55.0 56.3 58.7 1100-1383 bp 1.0 46.3
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAAGACTTGA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.3 57.8 1112-1395 bp 1.0 46.2
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAAGACTTGA GAAGGTCTACGGTTCGAGTC 33.3 55.0 56.3 57.5 1111-1394 bp 1.0 46.2
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAAGACTTG AGAAGGTCTACGGTTCGAGT 34.8 50.0 54.9 57.8 1112-1395 bp 1.0 46.2
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGACTTGAT AGAAGGTCTACGGTTCGAGT 32.0 50.0 56.8 57.8 1112-1395 bp 1.0 46.2