Hotspot

trnT-UGU-trnL-UAA

LSC · 46476-47254 · 779 bp

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Pi 0.0103
MarkerSeek score 64.90
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
779
variable_sites
12
pi
0.0103
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG GGGTTTCCATACCAAGGCTC 55.0 55.0 57.9 58.2 873-878 bp 1.0 78.8
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 840-845 bp 1.0 78.8
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT GGGTTTCCATACCAAGGCTC 50.0 55.0 57.8 58.2 905-910 bp 1.0 77.9
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 872-877 bp 1.0 77.8
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 834-839 bp 1.0 77.5