Hotspot

psbM-trnD-GUC

LSC · 29555-30470 · 916 bp

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Pi 0.018
MarkerSeek score 65.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
916
variable_sites
24
pi
0.018
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TTGACTGACTGTTTTTACGT TTCAATTGGTCAGAGCACCG 35.0 50.0 53.0 58.5 1061-1076 bp 1.0 43.2
psbM-trnD-GUC_p2 TTTGACTGACTGTTTTTACGT TTCAATTGGTCAGAGCACCG 33.3 50.0 53.9 58.5 1062-1077 bp 1.0 43.2
psbM-trnD-GUC_p3 TTTTGACTGACTGTTTTTACGT TTCAATTGGTCAGAGCACCG 31.8 50.0 54.8 58.5 1063-1078 bp 1.0 43.2
psbM-trnD-GUC_p4 TTGACTGACTGTTTTTACGT GTTCAATTGGTCAGAGCACC 35.0 50.0 53.0 57.0 1062-1077 bp 1.0 43.2
psbM-trnD-GUC_p5 TTGACTGACTGTTTTTACGT AGATCCCTTATTTCGCTGGG 35.0 50.0 53.0 57.1 1088-1103 bp 1.0 43.1