Hotspot

trnK-UUU-rps16

LSC · 4188-5018 · 831 bp

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Pi 0.0145
MarkerSeek score 56.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
831
variable_sites
18
pi
0.0145
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CTTCGCCCTAATCAAAATTAA 50.0 33.3 58.2 52.2 878-886 bp 1.0 42.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CTTCGCCCTAATCAAAATTAA 50.0 33.3 58.2 52.2 877-885 bp 1.0 42.9
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA CTTCGCCCTAATCAAAATTAA 50.0 33.3 58.3 52.2 887-895 bp 1.0 42.9
trnK-UUU-rps16_p4 TCAGTCGTGGTCTTCCAAAC CTTCGCCCTAATCAAAATTAA 50.0 33.3 57.8 52.2 963-971 bp 1.0 42.6
trnK-UUU-rps16_p5 CAGTCGTGGTCTTCCAAACT CTTCGCCCTAATCAAAATTAA 50.0 33.3 57.8 52.2 962-970 bp 1.0 42.6