Hotspot

trnH-GUG-psbA

LSC · 74-302 · 229 bp

Back to Ixora

Pi 0.064
MarkerSeek score 77.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
229
variable_sites
21
pi
0.064
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCAAGCTGAGCACAACAT 55.0 45.0 58.1 58.0 731-758 bp 1.0 84.4
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG GAGCTTAGTTTCCGTCTGGG 55.0 55.0 58.1 58.0 893-920 bp 1.0 83.5
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG GAGTGGGAGCTTAGTTTCCG 55.0 55.0 58.1 58.0 899-926 bp 1.0 83.5
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG GGAGCTTAGTTTCCGTCTGG 55.0 55.0 58.1 58.0 894-921 bp 1.0 83.5
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GGGAGCTTAGTTTCCGTCTG 55.0 55.0 58.1 58.0 895-922 bp 1.0 83.5