Hotspot

ycf1

SSC · 123104-128074 · 4971 bp

Pi 0.407

MarkerSeek score 75.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4971
variable_sites: 1758
pi: 0.407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGAGGAGGACCAAAAGGAT	GGACTGTCCCAAGGAAATCC	50.0	55.0	58.0	57.9	597 bp	0.5	58.6
ycf1_p2	TCGAGGAGGACCAAAAGGAT	TGACTGGGATGTATCGTGGA	50.0	50.0	58.0	57.8	1163 bp	0.5	58.4
ycf1_p3	TTTGCCCAACCTGTAGATCG	GGACTGTCCCAAGGAAATCC	50.0	55.0	57.9	57.9	890 bp	0.5	58.1
ycf1_p4	TTTGCCCAACCTGTAGATCG	TGACTGGGATGTATCGTGGA	50.0	50.0	57.9	57.8	1456 bp	0.5	57.9
ycf1_p5	TCGAGGAGGACCAAAAGGAT	ACGAGTTGCACCAACAGTTA	50.0	45.0	58.0	57.7	875 bp	0.5	57.1