Hotspot

rps15

SSC · 122428-122706 · 279 bp

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Pi 0.437
MarkerSeek score 75.50
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
279
variable_sites
107
pi
0.437
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 GTAAATCAGCAGCGACTCCA AATAATGGGAGCCGCTTGAG 50.0 50.0 58.0 58.0 1316 bp 0.5 59.7
rps15_p2 TAAATCAGCAGCGACTCCAC AATAATGGGAGCCGCTTGAG 50.0 50.0 58.0 58.0 1315 bp 0.5 59.7
rps15_p3 GTAAATCAGCAGCGACTCCA GAATAATGGGAGCCGCTTGA 50.0 50.0 58.0 58.0 1317 bp 0.5 59.7
rps15_p4 TAAATCAGCAGCGACTCCAC GAATAATGGGAGCCGCTTGA 50.0 50.0 58.0 58.0 1316 bp 0.5 59.7
rps15_p5 AACACCATGCATTGATGGGT AATAATGGGAGCCGCTTGAG 45.0 50.0 58.1 58.0 889 bp 0.5 59.3