Hotspot

ndhG

SSC · 117569-118099 · 531 bp

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Pi 0.462
MarkerSeek score 78.40
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
531
variable_sites
196
pi
0.462
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 CACATAAGCGCCCTAACCAT TTGATCACATCCGAGCGTTT 50.0 45.0 58.0 57.9 1562 bp 0.5 59.0
ndhG_p2 TCCAATAGCAGCTTCAGCAG TTGATCACATCCGAGCGTTT 50.0 45.0 58.0 57.9 1695 bp 0.5 59.0
ndhG_p3 AGTCCAATAGCAGCTTCAGC TTGATCACATCCGAGCGTTT 50.0 45.0 58.0 57.9 1697 bp 0.5 59.0
ndhG_p4 AGCGCCCTAACCATATTTCG TTGATCACATCCGAGCGTTT 50.0 45.0 58.1 57.9 1556 bp 0.5 58.3
ndhG_p5 GCGCCCTAACCATATTTCGA TTGATCACATCCGAGCGTTT 50.0 45.0 58.1 57.9 1555 bp 0.5 58.2